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80862
Ghost Dye™ UV 450 Viability Dye
Buffers & Dyes
Cellular Dyes

Ghost Dye™ UV 450 Viability Dye #80862

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Flow cytometric analysis of live and heat killed mouse bone marrow cells, combined and stained with Ghost Dye™ UV 450 Viability Dye. Viable gate is indicated.

Product Usage Information

1. Prepare the following reagents with reverse osmosis deionized (RODI) or equivalent grade water:

a. 1X PBS (azide- and protein/serum-free)

b. Incubation Buffer: Dissolve 0.5 g Bovine Serum Albumin (BSA) (#9998) in 100 ml 1X PBS. Store at 4°C.

2. Remove Ghost Dye™ from -20°C and bring to room temperature.

3. Collect cells by centrifugation and aspirate supernatant.

4. Wash cells by centrifugation in excess 1X PBS. Repeat if necessary.

5. Resuspend cells to a concentration of 1-10 x 106/mL in 1X PBS.

6. Centrifuge the Ghost Dye™ before opening then add 1 uL for each 1 mL of cell suspension and vortex immediately.

7. Incubate for 30 minutes at 4°C protected from light.

8. Wash by centrifugation in excess incubation buffer. Discard supernatant. Repeat.

9. Cells can then be stained, fixed and/or permeabilized based upon experimental design.

Ghost Dye™ UV 450 Viability Dye is excited by the UV (355 nm) laser line and has a peak emission of 450 nm that can be detected using a 450/50 band pass filter commonly used for detection of DAPI, Hoechst 33258, etc.

Storage

Store at -20°C desiccated and protected from light. This product is stable for 12 months. Aliquot to avoid excessive freeze-thaw cycles.

Product Description

Ghost Dye™ UV 450 Viability Dye is used to discriminate viable from non-viable mammalian cells in flow cytometry applications. Ghost Dye™ UV 450 Viability Dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Non-viable cells with loss of membrane integrity will react with significantly more Ghost Dye™ UV 450 Viability Dye than healthy cells in the same sample. Cells that exhibit increased fluorescence intensity can be excluded from analysis.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Ghost Dye is a registered trademark of Tonbo Biosciences.

To Purchase # 80862S
Product # Size Price
80862S
100 µl $ 84