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25690
Human Insulin-like Growth Factor II (hIGF-II)

Human Insulin-like Growth Factor II (hIGF-II) #25690

Coomassie Gel Image 1

The purity of recombinant hIGF-II was determined by SDS-PAGE of 1.5 µg reduced (+) and non-reduced (-) recombinant hIGF-II and staining overnight with Coomassie Blue.

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Bioactivity Image 2

FDC-P1 cells were cultured with 0 to 250 ng/mL of hIGF-II. Cell proliferation was assessed after 48 hours by measuring OD490.

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Western Blotting Image 3

Western blot analysis of extracts from NIH/3T3 cells untreated or treated with hIGF-II for 10 minutes, using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower).

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Recombinant human IGF-II was expressed in E. coli and is supplied in a lyophilized form. A greater than 95% purity was determined by SDS-PAGE. Endotoxin levels are less than or equal to 1 EU / 1 μg hIGF-II.

Purity:

> 95%

Bioactivity:

The bioactivity of recombinant hIGF-II was determined in a cell proliferation assay using FDC-P1 cells. The ED50 of each lot is between 10-20 ng/ml.

Working concentration of hIGF-II generally ranges from 1-100 ng/ml.

Storage:

Recombinant human IGF-II is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long term storage.

IGF-II is a potent cellular mitogen that is closely related to IGF-I (1). IGF-II is primarily produced by the liver and is frequently overexpressed in tumors (1,2). IGF-II binds to the IGF-IR, activating the AKT, mTOR, ERK, and JNK pathways (1). IGF-II signaling is regulated by several distinct mechanisms. First, IGF binding proteins (IGFBPs) bind to IGF-II and block interactions with the IGF-IR (1-3). Second, the IGF-IIR binds to and acts as a molecular trap for IGF-II (1-3). Lastly, the IGF2 gene is an imprinted gene, and loss of imprinting leads to increased IGF-II levels (1-3). Aberrant levels of IGF-II are associated with Wilms tumor, Beckwith-Wiedmann syndrome, and colorectal cancer (1,2).

  1. Pollak, M. (2008) Nat Rev Cancer 8, 915-28.
  2. Chitnis, M.M. et al. (2008) Clin Cancer Res 14, 6364-70.
  3. Sullivan, K.A. et al. (2008) Endocrinology 149, 5963-71.
Entrez-Gene Id
3481
Swiss-Prot Acc.
P01344
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.

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To Purchase # 25690S

Product Number Size Price
25690S 10 µg $65.00.0
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