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16483
Ibrutinib
Activators & Inhibitors
Chemical Modulators

Ibrutinib #16483

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Ibrutinib: Image 1

Western blot analysis of extracts from WEHI-231 cells, untreated (-) or treated (+) with combinations of the following treatments as indicated: H2O2 (100 mM, 20 min), Ibrutinib pretreatment (1 μM, 1 hr), using Phospho-Btk (Tyr223) (D9T6H) Rabbit mAb #87141, Btk (D3H5) Rabbit mAb #8547 and β-Actin (D6A8) Rabbit mAb #8457.

Ibrutinib: Image 2

Chemical structure of Ibrutinib.

Product Usage Information

Ibrutinib is supplied as a lyophilized powder. For a 10mM stock, reconstitute the 5 mg of powder in 1.14 ml DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used as a pretreatment at 1-10 μM for 1 hour prior to treating with a stimulator.

Storage

Store lyophilized or in solution at -20C, dessicated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid mulitple freeze/thaw cycles.

Product Description

Molecular Weight 440.5 g/mol
Purity

>99%

Molecular Formula

C25H24N6O2

CAS 936563-96-1
Solubility Soluble in DMSO at 200mg/ml and EtOH at 25mg/ml.

Background

Ibrutinib, also known as PCI-32765, is a small molecule inhibitor of Bruton's tyrosine kinase (Btk) (1,2). Research studies demonstrate that ibrutinib inhibits autophosphorylation of Btk as well as downstream targets such as PLCγ2 and ERK by binding to Cys481 in the active site (1,2). The inhibition of Btk also prevents NFκB DNA binding, reduces cell migration, proliferation and survival, inhibits DNA synthesis and induces apopotosis (1).

  1. Aalipour, A. and Advani, R.H. (2014) Ther Adv Hematol 5, 121-33.
  2. Chang, B.Y. et al. (2011) Arthritis Res Ther 13, R115.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 16483S
Product # Size Price
16483S
5 mg $ 157