View Featured Offers >>
53455
NSC-95397
Activators & Inhibitors
Chemical Modulators

NSC-95397 #53455

Citations (0)
Chemical structure of NSC-95397.

Product Usage Information

NSC-95397 is supplied as a lyophilized powder. For a 10 mM stock, reconstitute 5 mg of powder in 1.61 mL of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.

Storage

Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 310.4 g/mol
Purity >98%
Molecular Formula C14H14O4S2
CAS 93718-83-3
Solubility Soluble in DMSO at 15 mg/mL or ethanol at 1 mg/mL.

Background

NSC-95397 is a quinone-containing inhibitor of the cdc25 dual-specificity phosphatases responsible for regulating eukaryotic cell entry into mitosis. The primary substrate of cdc25A is CDK2, which allows progression through the G1/S and intra-S checkpoints; cdc25C activates CDK1 to overcome the G2/M checkpoint and allow mitotic entry (1). cdc25 phosphatase inhibition by NSC-95397 varies, with dissociation constants for cdc25A, -B, and -C of Ki = 32 nM, 96 nM, and 40 nM, respectively (2). NSC-95397 treatment of cancer cell lines leads to cell growth inhibition and cell cycle arrest at G2/M (1). Similar growth inhibition was seen in human hepatoma and breast cancer cells (3). Exposure of human erythrocytes to NSC-95397 triggers cell shrinkage, cell membrane scrambling, and eryptosis (4). The dual-specificity phosphatases MAP Kinase phosphatase 1 (MKP1) and MKP3 are likewise inhibited by NSC-95397, with NSC-95397 treatment followed by inhibited proliferation and reduced cell viability in a number of colon cancer cell lines (5,6).

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.
To Purchase # 53455
Cat. # Size Qty. Price
53455S
5 mg