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12808
PDBu (Phorbol 12,13-Dibutyrate)
Activators & Inhibitors
Chemical Modulators

PDBu (Phorbol 12,13-Dibutyrate) #12808

Citations (0)
Western blot analysis of extracts from HeLa cells serum-starved overnight, and untreated (-) or treated with PDBu (15 min; +) at the indicated concentrations, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (upper) or p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower).
Chemical structure of PDBu.

Product Usage Information

PDBu is supplied as a lyophilized powder. For a 2 mM stock, reconstitute the 1 mg in 990.9 µl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 20-2000 nM for 15-30 min. Soluble in DMSO or ethanol.

Storage

Store lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 504.61 g/mol
Purity >99%
Molecular Formula C28H40O8
CAS 37558-16-0
Solubility Souble in DMSO at 25mg/ml and EtOH at 20mg/ml.

Background

Phorbol 12, 13-Dibutyrate (PDBu) is a phorbol ester and a potent activator of protein kinase C (PKC). Frequently used in cancer research, PDBu is widely characterized to promote tumors in a variety of tissue types (1,2). PDBu treatment of cells can lead to activation of downstream PKC targets such as p44/42 MAPK and MARCKS (3-5). Although less potent than TPA, research has shown PDBu elicits less nonspecific binding (6,7) and is less hydrophobic allowing for easy wash out from cells in tissue culture (8).

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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To Purchase # 12808
Cat. # Size Qty. Price
12808S
1 mg