Western blot analysis of extracts from THP-1 cells differentiated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (80 nM, 18 hr) and then untransfected (-) or transfected with Poly(I:C) Sodium Salt (5 μg/ml, 6 hr; +), using Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb #4947 (upper), IRF-3 (D6I4C) XP® Rabbit mAb #11904 (middle), or β-Tubulin (D2N5G) Rabbit mAb #15115 (lower).
Poly(I:C) Sodium Salt is supplied as a lyophilized powder. To prevent denaturation, reconstitute in water containing physiological salt concentrations. Working concentrations and length of treatment can vary depending on the desired effect.
Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC. Aliquot to avoid multiple freeze/thaw cycles.
|Solubility||Soluble in water at 10 mg/ml with warming.|
Poly(I:C) Sodium Salt, also known as polyinosinic-polycytidylic acid sodium salt, is a synthetic analog to double-stranded RNA (dsRNA) and potent activator of Toll-like receptor 3 (TLR3). The Toll-like receptor (TLR) family plays an important role in the innate immune response (1). TLR3 functions as a receptor for dsRNA typically associated with viral infection (2). Binding of dsRNA, or the analog Poly(I:C), to TLR3 triggers activation of transcription factors NF-κB and IRF3 through the adaptor protein TICAM-1/TRIF (3,4). Studies have shown that combination treatment with Poly(I:C) can increase vaccine immunogenicity against viral and cancerous diseases (5,6).
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