Background
Ruxolitinib, also known as INCB018424, is a potent and selective ATP-competitive inhibitor of Jak1, Jak2, and Jak3 with an IC50 value of 2.7, 4.5, and 322 nM, respectively (1,2). This small molecule can block IL-6 signaling (IC50 = 281 nM) and proliferation of Jak2V617F+ Ba/F3 cells (IC50 = 127 nM) (3). The effect that Ruxolitinib has on Jak-signaling leads to the decreased circulation of the proinflammatory cytokines IL-6 and TNF-α, making it an important compound when studying myeloproliferative neoplasms (MPNs) (3).
This product has applications to SARS-CoV-2 research into the mechanisms of the Novel Coronavirus, which has caused the COVID-19 pandemic.
Molecular Formula
C17H18N6
Molecular Weight
306.4 g/mol
Purity
>98%
CAS
941678-49-5
Solubility
Soluble in DMSO at 28 mg/ml or ethanol at 15 mg/ml with slight warming.
Storage
Directions for Use
Ruxolitinib is supplied as a lyophilized powder. For a 15 mM stock, reconstitute 5 mg of powder in 1.09 ml of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.
Background References
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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