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Chemical Modulators

Ruxolitinib #83405

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Ruxolitinib: Image 1
Western blot analysis of extracts from HEL 92.1.7 cells, untreated (-) or treated with Ruxolitinib (300 nM, 24h; +), using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 (upper), Stat3 (D1B2J) Rabbit mAb #30835 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Ruxolitinib: Image 2
Chemical structure of Ruxolitinib.

Product Usage Information

Ruxolitinib is supplied as a lyophilized powder. For a 15 mM stock, reconstitute 5 mg of powder in 1.09 ml of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.


Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 306.4 g/mol
Purity >98%
Molecular Formula C17H18N6
CAS 941678-49-5
Solubility Soluble in DMSO at 28 mg/ml or ethanol at 15 mg/ml with slight warming.


Ruxolitinib, also known as INCB018424, is a potent and selective ATP-competitive inhibitor of Jak1, Jak2, and Jak3 with an IC50 value of 2.7, 4.5, and 322 nM, respectively (1,2). This small molecule can block IL-6 signaling (IC50 = 281 nM) and proliferation of Jak2V617F+ Ba/F3 cells (IC50 = 127 nM) (3). The effect that Ruxolitinib has on Jak-signaling leads to the decreased circulation of the proinflammatory cytokines IL-6 and TNF-α, making it an important compound when studying myeloproliferative neoplasms (MPNs) (3).

This product has applications to SARS-CoV-2 research into the mechanisms of the Novel Coronavirus, which has caused the COVID-19 pandemic.
  1. Eghtedar, A. et al. (2012) Blood 119, 4614-8.
  2. Verstovsek, S. (2009) Hematology Am Soc Hematol Educ Program, 636-42.
  3. Quintás-Cardama, A. et al. (2010) Blood 115, 3109-17.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
To Purchase # 83405
Cat. # Size Qty. Price
5 mg
$ 150