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83405
Ruxolitinib
Activators & Inhibitors
Chemical Modulators

Ruxolitinib #83405

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Western blot analysis of extracts from HEL 92.1.7 cells, untreated (-) or treated with Ruxolitinib (300 nM, 24h; +), using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 (upper), Stat3 (D1B2J) Rabbit mAb #30835 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Chemical structure of Ruxolitinib.

Product Usage Information

Ruxolitinib is supplied as a lyophilized powder. For a 15 mM stock, reconstitute 5 mg of powder in 1.09 ml of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.

Storage

Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight: 306.4 g/mol

Purity: >98%

Molecular Formula: C17H18N6

CAS: 941678-49-5

Solubility: Soluble in DMSO at 28 mg/ml or ethanol at 15 mg/ml with slight warming.

Background

Ruxolitinib, also known as INCB018424, is a potent and selective ATP-competitive inhibitor of Jak1, Jak2, and Jak3 with an IC50 value of 2.7, 4.5, and 322 nM, respectively (1,2). This small molecule can block IL-6 signaling (IC50 = 281 nM) and proliferation of Jak2V617F+ Ba/F3 cells (IC50 = 127 nM) (3). The effect that Ruxolitinib has on Jak-signaling leads to the decreased circulation of the proinflammatory cytokines IL-6 and TNF-α, making it an important compound when studying myeloproliferative neoplasms (MPNs) (3).

This product has applications to SARS-CoV-2 research into the mechanisms of the Novel Coronavirus, which has caused the COVID-19 pandemic.

  1. Eghtedar, A. et al. (2012) Blood 119, 4614-8.
  2. Verstovsek, S. (2009) Hematology Am Soc Hematol Educ Program, 636-42.
  3. Quintás-Cardama, A. et al. (2010) Blood 115, 3109-17.
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.

To Purchase # 83405S
Product # Size Price
83405S
5 mg $ 140