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Tyrphostin AG-490
Activators & Inhibitors
Chemical Modulators

Tyrphostin AG-490 #14704

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Tyrphostin AG-490: Image 1
Western blot analysis of extracts from NK-92 cells, serum-starved overnight and untreated (-) or treated with Human Interleukin-2 (hIL-2) #8907 (10 ng/ml, 15 min; +), either with or without Tyrphostin AG-490 pretreatment (1 hr) at the indicated concentrations, using Phospho-Jak3 (Tyr980/981) (D44E3) Rabbit mAb #5031, Jak3 (D1H3) Rabbit mAb #8827, Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145, Stat3 (79D7) Rabbit mAb #4904, and β-Actin (D6A8) Rabbit mAb #8457.
Tyrphostin AG-490: Image 2
Chemical structure of tyrphostin AG490

Product Usage Information

Tyrphostin AG-490 is supplied as a lyophilized powder. For a 50 mM stock, reconstitute the 10 mg in 679.58 μl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 10-100 μM for 1-24 hr.


Store lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 294.3 g/mol
Purity >99%
Molecular Formula C17H14N2O3
CAS 134036-52-5
Solubility Soluble in DMSO at 200mg/ml and EtOH at 16mg/ml.


Tyrphostin AG-490 is a membrane soluble protein tyrosine kinase inhibitor that primarily blocks Jak2 (IC50 = 10 μM) and Jak3 (IC50 = 20 μM) kinases (1,2). Research studies demonstrate that tyrphostin AG-490 inhibits proliferation and expansion of T cells (3). Unlike many other kinase inhibitors, tyrphostin AG-490 does not block a number of lymphocytic tyrosine kinases, such as Lck, Lyn, Btk, Syk, Jak1, Tyk2, or Src kinase (1,4). Additional studies indicate that tyrphostin AG-490 also selectively inhibits the EGFR (IC50 = 2 μM) and ErbB2 (IC50 = 13.5 μM) receptor tyrosine kinases (5).
  1. Meydan, N. et al. (1996) Nature 379, 645-8.
  2. Murakami, K. and Takagi, T. (1999) Pac Symp Biocomput, 171-81.
  3. Wang, L.H. et al. (1999) J Immunol 162, 3897-904.
  4. De Vos, J. et al. (2000) Br J Haematol 109, 823-8.
  5. Gazit, A. et al. (1991) J Med Chem 34, 1896-907.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
To Purchase # 14704
Cat. # Size Qty. Price
10 mg $ 115