Interested in promotions? | Click here >>
14255
Vinblastine
Activators & Inhibitors
Chemical Modulators

Vinblastine #14255

Reviews ()
Citations (0)
Vinblastine: Image 1
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Vinblastine (10 nM, 18 hr; +), using Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb #3377 (upper) or Histone H3 (D1H2) XP® Rabbit mAb #4499 (lower).
Vinblastine: Image 2
Western blot analysis of extracts from HeLa cells, untreated or treated with Vinblastine (18 hr) at the indicated concentrations, using Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb #3270 (upper), c-Jun (60A8) Rabbit mAb, #9165 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Product Usage Information

Vinblastine is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 5 mg in 550 μl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 10-1000 nM for 12-48 hr.

Storage

Store lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 909.05 g/mol
Purity >98%
Molecular Formula C46H58N4O9•H2SO4
CAS 143-67-9
Solubility Soluble in DMSO at 50mg/ml and H2O at 10mg/ml.

Background

Vinblastine is a vinca alkaloid derived from the plant Catharanthus roseus. Like other vinca alkaloids, it acts as a mitotic inhibitor blocking microtubule assembly in vitro (1,2). Investigators have demonstrated that vinblastine binds to and inhibits the addition of tubulin dimers to the assembly end of steady-state microtubules in a dose-dependent manner (Ki = ~178 nM) (1). This disruption of mitotic spindle causes mitotic arrest, inhibits cell proliferation, and induces apoptosis (1-3). Studies have suggested that high concentrations of vinblastine depolymerize microtubules, but this is not required for inhibition of cell proliferation (2). Treatment of cells with vinblastine has been shown to activate the SAPK/JNK pathway, leading to expression and phosphorylation of c-Jun (3,4) and phosphorylation of the anti-apoptotic proteins Bcl-2 and Bcl-xL (5,6).
  1. Jordan, M.A. et al. (1985) Cancer Res 45, 2741-7.
  2. Jordan, M.A. et al. (1991) Cancer Res 51, 2212-22.
  3. Kolomeichuk, S.N. et al. (2008) FEBS J 275, 1889-99.
  4. Duan, L. et al. (2007) Biochem Pharmacol 73, 481-90.
  5. Fan, M. et al. (2000) J Biol Chem 275, 29980-5.
  6. Poruchynsky, M.S. et al. (1998) Cancer Res 58, 3331-8.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
To Purchase # 14255
Cat. # Size Qty. Price
14255S
5 mg $ 80