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60332
Z-VAD(OMe)-FMK
Activators & Inhibitors
Chemical Modulators

Z-VAD(OMe)-FMK #60332

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Z-VAD(OMe)-FMK: Image 1

Chemical structure of Z-VAD(OMe)-FMK.

Z-VAD(OMe)-FMK: Image 2

Western blot analysis of extracts from Jurkat cells, untreated (-) or treated with Etoposide #2200 (25 μM, 5 hr; +), either with or without Z-VAD(OMe)-FMK pretreatment (1 hr) at the indicated concentrations, using Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb #9496, Caspase-8 (1C12) Mouse mAb #9746, Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb #5625, and PARP (46D11) Rabbit mAb #9532.

Product Usage Information

Z-VAD(OMe)-FMK is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 1 mg of powder in 213.9 μl of DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used as a pre-treatment at 5-100 μM for 1 hr.

Storage

Store lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 467.5 g/mol
Purity

>95%

Molecular Formula

C22H30FN3O7

CAS 187389-52-2
Solubility Soluble in DMSO at 5mg/ml.

Background

The synthetic peptide Z-VAD(OMe)-FMK is a cell permeable, irreversible pan-caspase inhibitor that displays non-cytotoxic effects (1-4). Z-VAD(OMe)-FMK prevents caspase-3 from adopting its active form and thereby blocks apoptosis (5). Research studies show that Z-VAD(OMe)-FMK also inhibits other enzymes, including cathepsin B, PNGase, and picornaviral 2A proteinases (6-8). Additional studies demonstrate that Z-VAD(OMe)-FMK can inhibit mitogen-induced T cell proliferation (2,9,10).

  1. Garcia-Calvo, M. et al. (1998) J Biol Chem 273, 32608-13.
  2. Lawrence, C.P. and Chow, S.C. (2012) Toxicol Appl Pharmacol 265, 103-12.
  3. Caserta, T.M. et al. (2003) Apoptosis 8, 345-52.
  4. Van Noorden, C.J. (2001) Acta Histochem 103, 241-51.
  5. Slee, E.A. et al. (1996) Biochem J 315 ( Pt 1), 21-4.
  6. Schotte, P. et al. (1999) FEBS Lett 442, 117-21.
  7. Misaghi, S. et al. (2006) Cell Death Differ 13, 163-5.
  8. Deszcz, L. et al. (2004) FEBS Lett 560, 51-5.
  9. Alam, A. et al. (1999) J Exp Med 190, 1879-90.
  10. Boissonnas, A. et al. (2002) Eur J Immunol 32, 3007-15.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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XP is a registered trademark of Cell Signaling Technology, Inc.
To Purchase # 60332S
Product # Size Price
60332S
1 mg $ 151