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Product Usage Information

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

AFP (3H8) Mouse mAb (Alexa Fluor® 555 Conjugate) recognizes endogenous levels of total AFP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant fragment of human AFP.

Product Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 555 fluorescent dye and tested in-house for direct immunofluorescent analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated AFP (3H8) Mouse mAb #3903.

Alpha-fetoprotein (AFP) is a 65 kDa glycoprotein found in the mammalian fetal liver, yolk sac, and GI tract. While AFP expression in adult cells is low, it is aberrantly expressed in adult liver cancer cells (1,2). The tumor suppressor gene p53 and β-catenin are both involved in the regulation of AFP expression. In normal adult cells, p53 binds to the repressor region of the AFP gene, thereby blocking transcription. Mutations in both p53 and β-catenin are associated with aberrant expression of AFP. Research studies have shown that elevated serum AFP levels are predictive of hepatocellular carcinoma (3).

1.  Meier, V. et al. (2006) Comp Hepatol 5, 2.

2.  Warnes, T.W. and Smith, A. (1987) Baillieres Clin Gastroenterol 1, 63-89.

3.  Peng, S.Y. et al. (2004) Int J Cancer 112, 44-50.

Entrez-Gene Id 174
Swiss-Prot Acc. P02771

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DRAQ5 is a registered trademark of Biostatus Limited.
The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

AFP (3H8) Mouse mAb (Alexa Fluor® 555 Conjugate)