Revision 1
Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
COX IV (3E11) Rabbit mAb (Alexa Fluor® 647 Conjugate) 7561 40 µl Rabbit IgG
PDI (C81H6) Rabbit mAb (Alexa Fluor® 647 Conjugate) 8463 40 µl Rabbit 
β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 647 Conjugate) 3624 40 µl Rabbit IgG
β-Actin (13E5) Rabbit mAb (Alexa Fluor® 647 Conjugate) 8584 40 µl Rabbit IgG
Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate) 2985 40 µl Rabbit IgG

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The Cellular Localization Alexa Fluor® 647 Conjugated Antibody Sampler Kit provides an economical means for identification of cellular organelles without the need for a fluorescent secondary antibody. This kit includes enough primary antibody to perform at least 20 IF-IC tests per antibody.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Background

Knowledge of the subcellular location of a protein may reveal the potential role it plays in the cell. The subcellular location of a protein of interest may be confirmed by colocalization with one of the organelle-specific antibodies in this kit.
β-Actin and β-tubulin are ubiquitous eukaryotic proteins that form the major component of the cellular cytoskeleton (1,2). Cytochrome c oxidase (COX) IV is part of the hetero-oligomeric enzyme found in the inner mitochondrial membrane (3). Protein disulfide isomerase (PDI) catalyzes the formation and isomerization of disulfide bonds in the endoplasmic reticulum (4).

  1. Herman, I.M. (1993) Curr Opin Cell Biol 5, 48-55.
  2. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.
  3. Ostermeier, C. et al. (1996) Curr Opin Struct Biol 6, 460-6.
  4. Ellgaard, L. and Ruddock, L.W. (2005) EMBO Rep 6, 28-32.

Background References

    Trademarks and Patents

    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.