FC-FP, FC-L
H
Endogenous
Mouse IgG2b kappa
#P11836
931
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:20 |
| Flow Cytometry (Live) | 1:20 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Product Description
Background
B-lymphocyte antigen CD20 (also known as MS4A1; Membrane-spanning 4-domains subfamily A member 1) is a cell surface phosphoprotein involved in the regulation of B cell activation and proliferation (1,2). It is commonly used as a marker to identify B cells and is expressed throughout B cell development, up until their differentiation into plasma cells. CD20 has no known ligand, and its expression and function are largely conserved between human and mouse (1-3). Evidence suggests that CD20 is necessary for store operated calcium (SOC) entry, which leads to elevated cytoplasmic calcium levels required for B cell activation (4,5). Anti-CD20 antibody immunotherapy depletes B cells by activation of the innate monocytic network and is a common treatment for B cell lymphomas, leukemias, and autoimmune diseases (6).
The 2H7 antibody is widely used to identify both normal and malignant B cells (7).
- Stashenko, P. et al. (1980) J Immunol 125, 1678-85.
- Tedder, T.F. et al. (1985) J Immunol 135, 973-9.
- Tedder, T.F. et al. (1988) J Immunol 141, 4388-94.
- Bubien, J.K. et al. (1993) J Cell Biol 121, 1121-32.
- Li, H. et al. (2003) J Biol Chem 278, 42427-34.
- Uchida, J. et al. (2004) J Exp Med 199, 1659-69.
- Liu, A.Y. et al. (1987) J Immunol 139, 3521-6.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
FC-FP: Flow Cytometry (Fixed/Permeabilized) FC-L: Flow Cytometry (Live)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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