Revision 4
Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

IHC-P, IF-F, IF-IC, FC-L

REACTIVITY:

M R Hm

SENSITIVITY:

Endogenous

MW (kDa):

Source/Isotype:

Rabbit IgG

UniProt ID:

#P31996

Entrez-Gene Id:

12514

Product Information

Product Usage Information

Application Dilution
Immunohistochemistry (Paraffin) 1:50 - 1:200
Immunofluorescence (Frozen) 1:400
Immunofluorescence (Immunocytochemistry) 1:400 - 1:800
Flow Cytometry (Live) 1:50

Storage

Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

CD68 (E3O7V) Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total CD68 protein. Staining of uncertain specificity in mouse endometrial epithelium has been observed by immunohistochemistry.

Species Reactivity:

Mouse, Rat, Hamster

Source / Purification

Monoclonal antibody is produced by immunizing animals with mouse CD68 recombinant protein and reacts with an epitope surrounding Gly113.

Product Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated CD68 (E3O7V) Rabbit mAb #97778.

Background

CD68 (macrosialin) is a heavily glycosylated transmembrane protein that is expressed by and commonly used as a marker for monocytes and macrophages (1,2). It is found on the plasma membrane, as well as endosomal and lysosomal membranes (1-3). It is proposed to bind OxLDL and has been observed as a homodimer (3,4).

  1. Rabinowitz, S.S. and Gordon, S. (1991) J Exp Med 174, 827-36.
  2. Holness, C.L. and Simmons, D.L. (1993) Blood 81, 1607-13.
  3. Ramprasad, M.P. et al. (1995) Proc Natl Acad Sci U S A 92, 9580-4.
  4. Ramprasad, M.P. et al. (1996) Proc Natl Acad Sci U S A 93, 14833-8.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

IHC-P: Immunohistochemistry (Paraffin) IF-F: Immunofluorescence (Frozen) IF-IC: Immunofluorescence (Immunocytochemistry) FC-L: Flow Cytometry (Live)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Revision 4
#51644

CD68 (E3O7V) Rabbit mAb (Alexa Fluor® 488 Conjugate)

Immunohistochemistry Image 1: CD68 (E3O7V) Rabbit mAb (Alexa Fluor® 488 Conjugate) Expand Image
Immunohistochemical analysis of paraffin-embedded mouse small intestine using CD68 (E3O7V) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green) and DAPI #4083 (blue).
Immunohistochemistry Image 2: CD68 (E3O7V) Rabbit mAb (Alexa Fluor® 488 Conjugate) Expand Image
Immunohistochemical analysis of paraffin-embedded A20 syngeneic tumor using CD68 (E3O7V) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green) and DAPI #4083 (blue).
Immunofluorescence Image 1: CD68 (E3O7V) Rabbit mAb (Alexa Fluor® 488 Conjugate) Expand Image
Confocal immunofluorescent analysis of fixed frozen mouse liver. Sections were labeled with LYVE-1 (E3L3V) Rabbit mAb #67538 (right, cyan) prior to blocking free secondary binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900. Sections were then labeled with CD68 (E3O7V) Rabbit mAb (Alexa Fluor® 488 Conjugate) (left, green), Ras (E4K9L) Rabbit mAb (Alexa Fluor® 555 Conjugate) #64520 (right, red), and DAPI #4083 (right, blue).
Immunofluorescence Image 2: CD68 (E3O7V) Rabbit mAb (Alexa Fluor® 488 Conjugate) Expand Image
Confocal immunofluorescent analysis of fixed frozen mouse spleen labeled with CD68 (E3O7V) Rabbit mAb (Alexa Fluor® 488 Conjugate) (left, green) and co-labeled with Ras (E4K9L) Rabbit mAb (Alexa Fluor® 555 Conjugate) #64520 (right, red) and DAPI #4083 (right, blue).
Immunofluorescence Image 1: CD68 (E3O7V) Rabbit mAb (Alexa Fluor® 488 Conjugate) Expand Image
Confocal immunofluorescent analysis of RAW 264.7 cells (left, positive) and Neuro-2a cells (right, negative) using CD68 (E3O7V) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green), DyLight 554 Phalloidin #13054 (red), and DAPI #4083 (blue).
Flow Cytometry Image 1: CD68 (E3O7V) Rabbit mAb (Alexa Fluor® 488 Conjugate) Expand Image
Flow cytometric analysis of BA/F3 cells (blue, negative) and RAW 264.7 cells treated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (100 ng/mL, 24 hr; green, positive) using CD68 (E3O7V) Rabbit mAb (Alexa Fluor® 488 Conjugate) (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate) #2975 (dashed lines).
Flow Cytometry Image 2: CD68 (E3O7V) Rabbit mAb (Alexa Fluor® 488 Conjugate) Expand Image
Flow cytometric analysis of RAW 264.7 cells, untreated (blue, low-expressing) or treated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (100 ng/mL, 24 hr; green, positive) using CD68 (E3O7V) Rabbit mAb (Alexa Fluor® 488 Conjugate) (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate) #2975 (dashed lines).