FC-FP, FC-L
M
Endogenous
Hamster (Armenian) IgG
#P37217
12515
Product Information
Product Usage Information
For optimal flow cytometry results, we recommend 0.5 μg of antibody per test.
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:40 |
| Flow Cytometry (Live) | 1:40 |
Storage
Specificity / Sensitivity
Species Reactivity:
Mouse
Source / Purification
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Product Description
Background
CD69, also known as Leu-23, is a type II transmembrane glycoprotein that is expressed on the surface of T cells, B cells, and NK cells (1,2). This phosphorylated disulfide-linked 28 to 32-kDa homodimer is constitutively expressed on a subset of thymocytes and platelets. It also acts as an activation antigen of lymphocytes, NK cells, neutrophils, and eosinophils (1-6). Studies have shown that stimulation of the T cell receptor (TCR) increases the expression of CD69 on the cell surface. The ability to detect the level of CD69 expression after TCR activation makes CD69 an ideal indicator of T cell activation (1).
The H1.2F3 antibody is widely used as a marker for T cell activation (7).
- Testi, R. et al. (1989) J Immunol 142, 1854-60.
- Marzio, R. et al. (1997) J Leukoc Biol 62, 349-55.
- Lanier, L.L. et al. (1988) J Exp Med 167, 1572-85.
- Testi, R. et al. (1988) J Immunol 141, 2557-63.
- Hartnell, A. et al. (1993) Immunology 80, 281-6.
- Gavioli, R. et al. (1992) Cell Immunol 142, 186-96.
- Sobel, E.S. et al. (1993) J Immunol 150, 673-82.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
FC-FP: Flow Cytometry (Fixed/Permeabilized) FC-L: Flow Cytometry (Live)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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