Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb (PE Conjugate) #12602

Product Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb #9496.

Storage:

Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.

Specificity / Sensitivity

Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb (PE Conjugate) recognizes human caspase-8 when cleaved at Asp391 (isoform A, Asp374 on isoform B). This antibody detects cleavage products containing the pro-domain with the p18 subunit, as well as the p18 subunit alone.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues adjacent to Asp391 of human caspase-8 protein.

Background

Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.

1. Muzio, M. et al. (1996) Cell 85, 817-27.
2. Boldin, M.P. et al. (1996) Cell 85, 803-15.
3. Fernandes-Alnemri, T. et al. (1996) Proc Natl Acad Sci U S A 93, 7464-9.

Pathways & Proteins

Explore pathways + proteins related to this product.

Select Your Pathway/Protein Apoptosis Regulation Death Receptor Signaling ErbB/HER Signaling Inhibition of Apoptosis Mitochondrial Control of Apoptosis Toll-Like Receptor Signaling Protein: Q14790

Database Links

Swiss-Prot Acc.: Q14790

Entrez-Gene Id: 841

View in PhosphoSitePlus^®