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12602
Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb (PE Conjugate)
Antibody Conjugates

Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb (PE Conjugate) #12602

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Citations (2)

Supporting Data

REACTIVITY
SENSITIVITY
MW (kDa)
Isotype Rabbit 

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb #9496.

Storage:

Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.

Specificity / Sensitivity

Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb (PE Conjugate) recognizes human caspase-8 when cleaved at Asp391 (isoform A, Asp374 on isoform B). This antibody detects cleavage products containing the pro-domain with the p18 subunit, as well as the p18 subunit alone.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues adjacent to Asp391 of human caspase-8 protein.

Background

Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.

  1. Muzio, M. et al. (1996) Cell 85, 817-27.
  2. Boldin, M.P. et al. (1996) Cell 85, 803-15.
  3. Fernandes-Alnemri, T. et al. (1996) Proc Natl Acad Sci U S A 93, 7464-9.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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