| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) (Alexa Fluor® 488 Conjugate) | 5407 | 40 µl | Rabbit | |
| GST-Tag (26H1) Mouse mAb (Alexa Fluor® 488 Conjugate) | 3368 | 40 µl | Mouse IgG2a | |
| HA-Tag (6E2) Mouse mAb (Alexa Fluor® 488 Conjugate) | 2350 | 40 µl | Mouse IgG1 | |
| Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 488 Conjugate) | 2279 | 40 µl | Mouse IgG2a | |
| Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 488 Conjugate) | 4878 | 40 µl | Mouse IgG1 | |
| Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate) | 2975 | 40 µl | Rabbit IgG |
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Description
The Epitope Tag Alexa Fluor® 488 Conjugated Antibody Sampler Kit provides an economical means to monitor the presence of proteins labeled with HA, GST, Myc, or DYKDDDDK (FLAG) tags without the need for a fluorescent secondary antibody.
Storage
Background
Epitope tags are useful for the labeling and detection of proteins using flow cytometry and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors (1). Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (2-4). Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect, and mammalian cell systems (5). DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion (6).
- Field, J. et al. (1988) Mol Cell Biol 8, 2159-65.
- Guan, K.L. and Dixon, J.E. (1991) Anal Biochem 192, 262-7.
- Davies, A.H. et al. (1993) Biotechnology (N Y) 11, 933-6.
- Yu, J. et al. (1998) Mol Cell Biol 18, 1379-87.
- Munro, S. and Pelham, H.R. (1984) EMBO J 3, 3087-93.
- Brizzard, B.L. et al. (1994) Biotechniques 16, 730-5.
Background References
Trademarks and Patents
Limited Uses
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