Revision 6

#67090

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
IP

Reactivity:
All

Sensitivity:
Transfected Only

Source/Isotype:
Mouse IgG2b

UniProt ID:
#P42212

Product Usage Information

Application Dilution
Immunoprecipitation 1:20

Storage

Supplied in PBS Buffer (pH 7.2), 0.1% Tween® 20. Store at 4°C. Do not aliquot the antibody.

Specificity/Sensitivity

GFP (5G4) Mouse Monoclonal Antibody (Magnetic Bead Conjugate) detects native GFP and YFP-tagged proteins exogenously expressed in cells, but does not detect denatured GFP. This antibody conjugate does not detect RFP-tagged proteins. GFP and YFP tags add approximately 27 kDa to the molecular weight of the fusion protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with native GFP.

Description

This Cell Signaling Technology antibody is immobilized by the covalent reaction of formylbenzamide-modified antibody with hydrazide-activated magnetic bead. GFP (5G4) Mouse mAb (Magnetic Bead Conjugate) is useful for immunoprecipitation assays of GFP-tagged recombinant proteins. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated GFP (5G4) Mouse mAb #55494.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineage, and defines subcellular protein localization (1).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

IP: Immunoprecipitation

Cross-Reactivity Key

All: All Species Expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 6

Cell Signaling Technology Logo
Immunoprecipitation of GFP protein from HeLa/LC3 GFP ATG9A KO cell extracts. Lane 1 is Mouse IgG (Magnetic Bead Conjugate) #5873, lane 2 is GFP (5G4) Mouse mAb (Magnetic Bead Conjugate), and lane 3 is 10% input. Western blot analysis was performed using GFP (D5.1) Rabbit mAb #2956. Cells were kindly provided by Dr. Josh Anderson (Brigham Young University) and used with permission.
Immunoprecipitation Image 1: GFP (5G4) Mouse Monoclonal Antibody (Magnetic Bead Conjugate)
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.