FC-FP
H
Endogenous
Rabbit IgG
#Q02535
3399
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Dog, Rabbit
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ID3 protein.
Product Description
Background
Inhibitor of DNA-binding/Differentiation (ID) proteins are a family of proteins that function to repress the activity of basic helix-loop-helix (bHLH) transcription factors. There are four known ID proteins in humans (ID1-4), all of which contain a helix-loop-helix domain but lack a basic DNA binding domain. Heterodimerization with bHLH transcription factors therefore functions to sequester bHLH proteins and prevent their binding to DNA (1). ID proteins play important functional roles in development, primarily by inhibiting premature differentiation of stem/progenitor cells (1,2). ID3 plays an important role in immune system development where it has been shown to repress E2A-mediated differentiation of T cells (3). Studies in mouse models have shown that homozygous deletion of ID3 disrupts regulatory T cell differentiation (4) and leads to development of γδ T cell lymphoma (5). Outside of the hematopoietic compartment, ID3 was shown to repress MyoD, implicating ID3 in TGFβ-mediated muscle repair (6). Similarly, research studies have shown that ID3 suppresses p21 in colon cancer cells, a function that is purported to promote the self-renewal capacity of putative cancer-initiating cells (7).
- Yokota, Y. (2001) Oncogene 20, 8290-8.
- Hong, S.H. et al. (2011) J Cell Sci 124, 1445-52.
- Miyazaki, M. et al. (2011) Nat Immunol 12, 992-1001.
- Maruyama, T. et al. (2011) Nat Immunol 12, 86-95.
- Li, J. et al. (2010) Blood 116, 5615-21.
- Clever, J.L. et al. (2010) Am J Physiol Cell Physiol 298, C1087-99.
- O'Brien, C.A. et al. (2012) Cancer Cell 21, 777-92.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
FC-FP: Flow Cytometry (Fixed/Permeabilized)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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