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REACTIVITY SENSITIVITY MW (kDa) Isotype
Rabbit 

Product Usage Information

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

IL-4 (D19A10) Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total IL-4 protein.


Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human IL-4 protein.

Product Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometric analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated IL-4 (D19A10) Rabbit mAb #12227.


Interleukin-4 (IL-4) is a cytokine secreted by activated T cells, basophils, and mast cells (1,2). While it contributes to many immunomodulatory responses, it is mainly recognized as the cytokine responsible for eliciting differentiation of naive T cells into Th2 lineage cells that are defined by their secretion of IL-4, IL-5, and IL-10 (3). In addition, IL-4 contributes to immunoglobulin class switching by inducing the production of IgE from B cells (4,5). IL-4 acts through the IL-4 receptor, leading to tyrosine phosphorylation and activation of the Stat6 transcription factor (6).


1.  Yokota, T. et al. (1986) Proc Natl Acad Sci USA 83, 5894-8.

2.  Grabstein, K. et al. (1986) J Exp Med 163, 1405-14.

3.  Kopf, M. et al. (1993) Nature 362, 245-8.

4.  Kotowicz, K. and Callard, R.E. (1993) Eur J Immunol 23, 2250-6.

5.  Thyphronitis, G. et al. (1989) Proc Natl Acad Sci USA 86, 5580-4.

6.  Hou, J. et al. (1994) Science 265, 1701-1706.


Entrez-Gene Id 3565
Swiss-Prot Acc. P05112


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
The Alexa Fluor dye conjugates in this product are sold under license from Life Technologies Corporation, for research use only excluding use in combination with DNA microarrays and high content screening (HCS).
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, including use with HCS or other automated imaging applications but excluding use in combination with DNA microarrays. The buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

13351
IL-4 (D19A10) Rabbit mAb (Alexa Fluor® 488 Conjugate)