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REACTIVITY SENSITIVITY MW (kDa) Isotype
Rabbit 

Product Usage Information

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.

Specificity / Sensitivity

IL-4 (D19A10) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total IL-4 protein.


Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human IL-4 protein.

Product Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated IL-4 (D19A10) Rabbit mAb #12227.


Interleukin-4 (IL-4) is a cytokine secreted by activated T cells, basophils, and mast cells (1,2). While it contributes to many immunomodulatory responses, it is mainly recognized as the cytokine responsible for eliciting differentiation of naive T cells into Th2 lineage cells that are defined by their secretion of IL-4, IL-5, and IL-10 (3). In addition, IL-4 contributes to immunoglobulin class switching by inducing the production of IgE from B cells (4,5). IL-4 acts through the IL-4 receptor, leading to tyrosine phosphorylation and activation of the Stat6 transcription factor (6).


1.  Yokota, T. et al. (1986) Proc Natl Acad Sci USA 83, 5894-8.

2.  Grabstein, K. et al. (1986) J Exp Med 163, 1405-14.

3.  Kopf, M. et al. (1993) Nature 362, 245-8.

4.  Kotowicz, K. and Callard, R.E. (1993) Eur J Immunol 23, 2250-6.

5.  Thyphronitis, G. et al. (1989) Proc Natl Acad Sci USA 86, 5580-4.

6.  Hou, J. et al. (1994) Science 265, 1701-1706.


Entrez-Gene Id 3565
Swiss-Prot Acc. P05112


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

12884
IL-4 (D19A10) Rabbit mAb (PE Conjugate)