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Myc-Tag (9B11) Mouse Monoclonal Antibody (trFluor Europium Cryptate Conjugate) #93510

    Product Specifications

    REACTIVITY All
    SENSITIVITY Transfected Only
    MW (kDa)
    Source/Isotype Mouse IgG2a kappa
    Species Cross-Reactivity Key:
    • All-All Species Expected 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody is the trFluorTM Europium Cryptate version of the unconjugated Myc-Tag (9B11) Mouse mAb #2276 and is expected to detect transfected cell lysate or recombinant proteins containing the Myc tag. Time-resolved (TR) fluorescence resonance energy transfer (FRET), or TR-FRET,  data were generated using the conjugated antibody.

    Fluorescent Properties

    • ← Excitation: 305 nm ← Emission: 617 nm

    Product Usage Information

    trFluor™ Europium Cryptate conjugated antibodies are ideal for protein-protein interaction assays, pair-based immunoassay technologies, or high-throughput screening where greater sensitivity and quantitative detection is needed. The long fluorescent decay time of Europium Cryptate results in improved signal to background over fluorescent dye conjugates. Methods utilizing Europium Cryptate conjugated antibodies include, but are not limited to, TR-FRET, where Europium Cryptate-labeled donor antibodies are paired with red fluorophore-labeled acceptor antibodies, such as Alexa Fluor® 647 or APC. Please contact us if you require an acceptor antibody clone conjugated to a red dye at a custom concentration, a carrier-free formulation, or a more customized packaging solution.

    Note: Addition of potassium fluoride (KF) in the assay dilution buffer range of 40-400 mM is recommended for enhanced fluorescent signal. Optimal dilutions and working concentrations of both the conjugated antibodies and the KF should be determined by the end user.

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Specificity / Sensitivity

    Myc-Tag (9B11) Mouse Monoclonal Antibody (trFluor™ Europium Cryptate Conjugate) detects exogenously expressed proteins containing the Myc epitope tag. This antibody conjugate recognizes the Myc tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. This antibody conjugate detects exogenously expressed Myc-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated. This antibody conjugate may cross-react with c-myc protein and weakly cross-react with a protein of unknown origin at approximately 90 kDa.

    Species Reactivity:

    All Species Expected

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).

    Background

    Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

    The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1).
    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    trFluor is a trademark of AAT Bioquest, Inc.
    trFluor is manufactured by AAT Bioquest, Inc.
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