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REACTIVITY SENSITIVITY MW (kDa) Isotype
Rabbit 

Product Usage Information

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

OX40 (D4N3K) Rabbit mAb (Pacific Blue™ Conjugate) recognizes endogenous levels of total OX40 protein. This antibody binds the extracellular domain of human OX40 protein.


Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln156 of human OX40 protein.

Product Description

This Cell Signaling Technology antibody is conjugated to Pacific Blue™ fluorescent dye and tested in-house for direct flow cytometry in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody OX40 (D4N3K) Rabbit mAb  #15122.


OX40 (TNFRSF4, CD134) is a member of the tumor necrosis factor (TNF) receptor superfamily that regulates T cell activity and immune responses. The OX40 protein contains four cysteine rich domains, a transmembrane domain, and a cytoplasmic tail containing a QEE motif (1,2). OX40 is primarily expressed on activated CD4+ and CD8+ T-cells, while the OX40 ligand (OX40L, TNFSF4, CD252) is predominantly expressed on activated antigen presenting cells (3-7). The engagement of OX40 with OX40L leads to the recruitment of TNF receptor-associated factors (TRAFs) and results in the formation of a TCR-independent signaling complex. One component of this complex, PKCθ, activates the NF-κB pathway (2,8). OX40 signaling through Akt can also enhance TCR signaling directly (9). Research studies indicate that the OX40L-OX40 pathway is associated with inflammation and autoimmune diseases (10). Additional research studies show that OX40 agonists augment anti-tumor immunity in several cancer types (11,12).


1.  Croft, M. (2010) Annu Rev Immunol 28, 57-78.

2.  So, T. and Croft, M. (2012) Front Immunol 3, 133.

3.  Paterson, D.J. et al. (1987) Mol Immunol 24, 1281-90.

4.  Mallett, S. et al. (1990) EMBO J 9, 1063-8.

5.  Dürkop, H. et al. (1995) Br J Haematol 91, 927-31.

6.  Godfrey, W.R. et al. (1994) J Exp Med 180, 757-62.

7.  Al-Shamkhani, A. et al. (1997) J Biol Chem 272, 5275-82.

8.  So, T. et al. (2011) Proc Natl Acad Sci U S A 108, 2903-8.

9.  So, T. and Croft, M. (2013) Front Immunol 4, 139.

10.  Gough, M.J. and Weinberg, A.D. (2009) Adv Exp Med Biol 647, 94-107.

11.  Weinberg, A.D. et al. (2011) Immunol Rev 244, 218-31.

12.  Linch, S.N. et al. (2015) Front Oncol 5, 34.


Entrez-Gene Id 7293
Swiss-Prot Acc. P43489


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Pacific Blue is a trademark of Molecular Probes, Inc.

15148
OX40 (D4N3K) Rabbit mAb (Pacific Blue™ Conjugate)