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PhosphoSitePlus® Resource

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REACTIVITY SENSITIVITY MW (kDa) Isotype
Rabbit 

Product Usage Information

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

PD-1 (D3W4U) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total PD-1 protein. This antibody binds the intracellular domain of human PD-1 protein.


Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala249 of human PD-1 protein.

Product Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated PD-1 (D3W4U) Rabbit mAb #15121.


The programmed cell death 1 protein (PD-1, PDCD1, CD279) is a member of the CD28 family of immunoreceptors that regulate T cell activation and immune responses (1-3). The PD-1 protein contains an extracellular Ig V domain, a transmembrane domain, and a cytoplasmic tail that includes an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an immunoreceptor tyrosine-based switch motif (ITSM). PD-1 is activated by the cell surface ligands PD-L1 and PD-L2 (4). Upon activation, PD-1 ITIM and ITSM phosphorylation leads to the recruitment of the protein tyrosine phosphatases SHP-1 and SHP-2, which suppress TCR signaling (5-7). In addition to activated T-cells, PD-1 is expressed in activated B-cells and monocytes, although its function in these cell types has not been fully characterized (8). The PD-1 pathway plays an important role in immune tolerance (3); however, research studies show that cancer cells often adopt this pathway to escape immune surveillance (9). Consequently, blockade of PD-1 and its ligands is proving to be a sound strategy for neoplastic intervention (10).


1.  Ishida, Y. et al. (1992) EMBO J 11, 3887-95.

2.  Shinohara, T. et al. (1994) Genomics 23, 704-6.

3.  Nishimura, H. et al. (1999) Immunity 11, 141-51.

4.  Freeman, G.J. et al. (2000) J Exp Med 192, 1027-34.

5.  Yokosuka, T. et al. (2012) J Exp Med 209, 1201-17.

6.  Sheppard, K.A. et al. (2004) FEBS Lett 574, 37-41.

7.  Chemnitz, J.M. et al. (2004) J Immunol 173, 945-54.

8.  Thibult, M.L. et al. (2013) Int Immunol 25, 129-37.

9.  Dong, H. et al. (2002) Nat Med 8, 793-800.

10.  Topalian, S.L. et al. (2012) Curr Opin Immunol 24, 207-12.


Entrez-Gene Id 5133
Swiss-Prot Acc. Q15116


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
The Alexa Fluor dye conjugates in this product are sold under license from Life Technologies Corporation, for research use only excluding use in combination with DNA microarrays and high content screening (HCS).
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, including use with HCS or other automated imaging applications but excluding use in combination with DNA microarrays. The buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

15142
PD-1 (D3W4U) Rabbit mAb (Alexa Fluor® 647 Conjugate)