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PhosphoSitePlus® Resource

  • Additional protein information
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REACTIVITY SENSITIVITY MW (kDa) Isotype
Rabbit 

Product Usage Information

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb  (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of HS1 protein only when phosphorylated at Tyr397.


Species predicted to react based on 100% sequence homology: Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr405 of mouse HS1 protein. This site corresponds to Tyr397 of human HS1 protein.

Product Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb #8714.


HS1 (HCLS1, LckBP1, p75) is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (1,2). HS1 contains four cortactin repeats and a single SH3 domain (2). This intracellular protein is phosphorylated following immune receptor activation, which promotes recruitment of HS1 to the immune synapse (3-5). Phosphorylation of HS1 is required to regulate actin dynamics and provide docking sites for many other signaling molecules, such as Vav1 and PLCγ1 (6). HS1 also plays an important role in platelet activation (7).


1.  Kitamura, D. et al. (1989) Nucleic Acids Res 17, 9367-79.

2.  Kitamura, D. et al. (1995) Biochem Biophys Res Commun 208, 1137-46.

3.  Suzuki, H. et al. (1997) J Immunol 159, 5881-8.

4.  Hata, D. et al. (1994) Immunol Lett 40, 65-71.

5.  Yamanashi, Y. et al. (1993) Proc Natl Acad Sci USA 90, 3631-5.

6.  Gomez, T.S. et al. (2006) Immunity 24, 741-52.

7.  Kahner, B.N. et al. (2007) Blood 110, 2449-56.


Entrez-Gene Id 3059
Swiss-Prot Acc. P14317


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

13090
Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate)