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PhosphoSitePlus® Resource

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REACTIVITY SENSITIVITY MW (kDa) Isotype
Rabbit 

Product Usage Information

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) recognizes endogenous levels of Rb protein only when phosphorylated at Ser807, Ser811, or at both sites. This antibody does not cross-react with Rb phosphorylated at Ser608.


Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser807/811 of human Rb protein.

Product Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 549 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb #8516.


The retinoblastoma tumor suppressor protein Rb regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).


1.  Sherr, C.J. (1996) Science 274, 1672-7.

2.  Nevins, J.R. (1992) Science 258, 424-9.

3.  Welch, P.J. and Wang, J.Y. (1993) Cell 75, 779-90.

4.  Hu, Q.J. et al. (1990) EMBO J 9, 1147-55.

5.  Knudsen, E.S. and Wang, J.Y. (1997) Mol Cell Biol 17, 5771-83.

6.  Lundberg, A.S. and Weinberg, R.A. (1998) Mol Cell Biol 18, 753-61.

7.  Connell-Crowley, L. et al. (1997) Mol Biol Cell 8, 287-301.

8.  Kitagawa, M. et al. (1996) EMBO J 15, 7060-9.

9.  Geng, Y. et al. (2001) Proc Natl Acad Sci USA 98, 194-9.


Entrez-Gene Id 5925
Swiss-Prot Acc. P06400


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

8880
Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate)