FC-FP
H
Endogenous
Rabbit IgG
#P50591
8743
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys60 of human TRAIL, within the extracellular region of the protein.
Product Description
Background
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL), also referred to as Apo2 ligand, first identified based on its sequence homology to TNF and Fas/Apo ligand is a member of the TNF family of cytokines and either exists as a type II membrane or soluble protein (1,2). TRAIL induces apoptosis in a variety of transformed cell lines and plays a role in anti-tumor and anti-viral immune surveillance (3). TRAIL signals via binding with death receptors DR4 (TRAIL-R1) (4) and DR5 (TRAIL-R2) (5-8) which can trigger apoptosis as well as NF-κB activation (7,9). Death domains on these receptors leads to the recruitment of a death-induced signaling complex (DISC) leading to caspase-8 and subsequent caspase-3 activation. In addition, TRAIL binds with decoy receptors DcR1 (TRAIL-R3) (6,8,10,11) and DcR2 (TRAIL-R4, TRUNDD) (12,13) which lack the functional cytoplasmic death domain antagonizing TRAIL-induced apoptosis. Osteoprotegerin (OPG) has also been identified as receptor capable of inhibiting TRAIL-induced apoptosis (14). The selectivity of soluble TRAIL at triggering apoptosis in transformed cells as compared to normal cells has led to its investigation as a potential cancer therapeutic (15,16).
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- Pitti, R.M. et al. (1996) J Biol Chem 271, 12687-90.
- Almasan, A. and Ashkenazi, A. Cytokine Growth Factor Rev 14, 337-48.
- Pan, G. et al. (1997) Science 276, 111-3.
- Walczak, H. et al. (1997) EMBO J 16, 5386-97.
- MacFarlane, M. et al. (1997) J Biol Chem 272, 25417-20.
- Chaudhary, P.M. et al. (1997) Immunity 7, 821-30.
- Schneider, P. et al. (1997) FEBS Lett 416, 329-34.
- Shetty, S. et al. (2002) Apoptosis 7, 413-20.
- Sheridan, J.P. et al. (1997) Science 277, 818-21.
- Degli-Esposti, M.A. et al. (1997) J Exp Med 186, 1165-70.
- Pan, G. et al. (1998) FEBS Lett 424, 41-5.
- Marsters, S.A. et al. (1997) Curr Biol 7, 1003-6.
- Kelley, S.K. et al. (2001) J Pharmacol Exp Ther 299, 31-8.
- Walczak, H. et al. (1999) Nat Med 5, 157-63.
- Ashkenazi, A. et al. (1999) J Clin Invest 104, 155-62.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
FC-FP: Flow Cytometry (Fixed/Permeabilized)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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