View Featured Offers >>
10X Wash Buffer, Phosphate Buffered Saline (PBS)
Buffers & Dyes

10X Wash Buffer, Phosphate Buffered Saline (PBS) #12528

Reviews ()
Citations (0)

Product Usage Information

Dilute to a 1X solution using ddH2O. Once diluted to 1X, store at 4°C and use within one month.


Store 10X buffer at room temperature. Undiluted 10X buffer contains 0.05% sodium azide as a preservative and is stable for 12 months. Peroxidase-based fluorescence assays may be negatively affected by sodium azide (1).

Product Description

10X Wash Buffer, Phosphate Buffered Saline (PBS) is for use in immunfluorescence and flow cytometry assays. Cell Signaling Technology recommends using this wash buffer according to our protocols for IF and FLOW approved antibodies to ensure accurate and reproducible results.
1X Wash Buffer contains 137 mM NaCl, 2.6 mM KCl, 10 mM Na2HPO4, 1.76 mM KH2PO4, 0.005% sodium azide, pH 8.0. The product is free of calcium and magnesium salts.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.
To Purchase # 12528
Cat. # Size Qty. Price
200 ml
$ 134