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4084
DRAQ5®
Buffers & Dyes
Cellular Dyes

DRAQ5® #4084

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Citations (8)
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  1. IF
  2. F
Immunofluorescence Image 1: DRAQ5®

Confocal immunofluorescent analysis of HeLa cells using COX IV (3E11) Rabbit mAb #4850 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).

Flow Cytometry Image 1: DRAQ5®

Flow cytometric analysis of Jurkat cells using Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb (PE Conjugate) #29237 versus DRAQ5® (DNA content).

Product Image 1: DRAQ5®

Absorption and fluorescence emission spectra of DRAQ5® in aqueous solution.

Product Usage Information

Please follow CST’s recommended IF and Flow protocols. For both applications, following secondary detection:

Flow Cytometry: Centrifuge cells and resuspend in 0.5 ml DRAQ5® diluted 1:5000 (1 µM) in Incubation Buffer. Incubate for 5 min at room temperature in the dark before analyzing cells on flow cytometer.

Immunofluorescence: Rinse samples twice in PBS for five minutes each. Dilute DRAQ5® 1:1000 (5 µM) in PBS and incubate for 5 minutes at room temperature in the dark. Rinse samples once in PBS, coat coverslips with anti-fade reagent and examine immediately using appropriate excitation wavelength.

Storage

Store at 4°C protected from light.

Background

DRAQ5®, 1,5-bis{[2-(di-methylamino) ethyl]amino}-4, 8-dihydroxyanthracene-9,10-dione, is a cell permeable far-red fluorescent DNA dye that can be used in live or fixed cells. This dye can be used in combination with GFP or FITC labels. DRAQ5® has been used to examine cellular DNA in flow cytometry and fluorescent microscopy applications (1-3).

  1. Smith, P.J. et al. (2000) Cytometry 40, 280-91.
  2. Wiltshire, M. et al. (2000) Cytometry 39, 217-23.
  3. Smith, P.J. et al. (1999) J Immunol Methods 229, 131-9.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
DRAQ5 is a registered trademark of Biostatus Limited.
To Purchase # 4084S
Product # Size Price
4084S
50 µl $ 214
4084L
200 µl $ 443