DRAQ7™ #7406

• Exλ_max599/644 nm
• Emλ_max678/694 nm
• DRAQ7™ may be excited by a wide range of wavelengths from 488-647 nm. Despite low absorbance at 488 nm, this excitation source allows for convenient combination with FITC and PE conjugates as well as EGFP.

Flow Cytometry: Prepare cells for DRAQ7™ staining by resuspending cells in PBS at a concentration of no more than 5 x 10⁵ cells/ml. For each 0.5 ml of cell suspension, dilute DRAQ7™ 1:100 by adding 5 μl to suspended cells, achieving a final concetration of 3 μM. Gently mix and incubate for 10 minutes on ice. Cells should be analyzed directly in the presence of DRAQ7™.

Formulation:

DRAQ7™ is provided as a 0.3 mM stock.

Storage:

Store at 4°C, protected from light. Do not freeze.

DRAQ7™ is a far-red fluorescent DNA dye that only stains the nuclei in dead and permeabilized cells. It can be used in combination with GFP, FITC, and PE labels.

Stratikopoulos, E.E. et al. (2015) Cancer Cell 27, 837-51.

Ekert, J.E. et al. (2014) PLoS One 9, e92248.

Data sheet (165KB) Certificate of Analysis

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