Chemical structure of DTT.
DTT is supplied as a lyophilized powder. To prepare a 1 M stock solution, reconstitute the 192.8 mg supplied in the product tube in 1.12 ml ddH2O. The dissolved DTT will increase the final volume to approximately 1.25 ml.
Store lyophilized at 4ºC, desiccated. In lyophilized form, the chemical is stable for 12 months. Once in solution, store at -20ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
Dithiothreitol (DTT) from Cell Signaling Technology is offered in a convenient 192.8 mg lyophilized format, allowing for preparation of a fresh stock solution. This DTT reagent contains no detectable DNase or RNase activity and is suitable for use in molecular biology or protein biochemistry applications that require reduction of protein disulfide bonds.
SDS-PAGE sample buffers are routinely supplemented with 10-50 mM DTT to cleave protein disulfide bonds. Lower concentrations of DTT are routinely used to stabilize enzymes or other proteins that posses free sulfhydryl groups, which is useful in chromatin immunoprecipitation (ChIP) assays.
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