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14166
Hematoxylin
Buffers & Dyes
Cellular Dyes

Hematoxylin #14166

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Hematoxylin: Image 1

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Claudin-1 (D5H1D) XP® Rabbit mAb #13255 (brown) followed by a counterstain with Hematoxylin (blue).

Hematoxylin: Image 2

Chemical structure of Hematoxylin.

Product Usage Information

Hematoxylin is a blue nuclear counterstain for use after the completion of immunohistochemical assays. It yields crisp nuclear staining with superior contrast with SignalStain® DAB Substrate Kit #8059 and is also compatible with SignalStain® Mounting Medium #14177.

A) Solutions and Reagents

Supplied Reagents

1. Hematoxylin #14166

Additional Reagents (Not Supplied)

1. Acid Rinse Solution: To prepare 100ml, add 2ml of glacial acetic acid to 98 ml of dH20.

2. Bluing Solution: To prepare 100ml, add 1.5 ml of 30% ammonium hydroxide (NH4OH) to 98.5ml of 70% ethanol

3. SignalStain® Mounting Medium #14177

B) Nuclear Counterstaining

1. Rinse slides in tap water.

2. Completely submerse slides in Hematoxylin #14166 and incubate for 1-5 minutes.

3. Wash slides with tap water until rinse water is clear.

4. Immerse slides 10 times in acid rinse solution.

5. Rinse slides 10 times in tap water.

6. Immerse slides for 1 minute in bluing solution

7. Rinse slides 10 times in tap water.

8. Mount sections with SignalStain® Mounting Medium #14177.

Storage

Supplied as a working solution. Store at room temperature. Product is stable for 12 months upon receipt when stored properly.

Product Description

Hematoxylin is a blue nuclear counterstain for use in immunohistochemical assays. It yields crisp staining detail with superior contrast when used in conjunction with SignalStain® DAB Substrate Kit #8059. It is also compatible with SignalStain® Mounting Medium #14177.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
To Purchase # 14166S
Product # Size Price
14166S
500 ml $ 177