Product Information
Following specimen preparation:
1. Permeabilize the cells in Permeabilization Buffer for 5 minutes at room temperature.
2. Rinse three times in PBS for 5 minutes each.
3. Apply 3–4 drops of Image-iT™ FX Signal Enhancer (or sufficient volume to cover the cells) and incubate for 30 minutes at room temperature.
4. Rinse three times with PBS for 5 minutes each.
5. Block specimen in Blocking Buffer for 60 minutes.
6. While blocking, prepare primary antibody by diluting as indicated on datasheet in Antibody Dilution Buffer.
7. Aspirate blocking solution, apply diluted primary antibody.
8. Incubate overnight at 4°C.
9. Rinse three times in PBS for 5 minutes each.
10. Coverslip slides with Prolong® Gold Antifade Reagent.
11. For best results, examine specimens immediately using appropriate excitation wavelength. For long-term storage, store slides flat at 4°C protected from light.
Store at room temperature. This product is stable for 12 months.
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| Cat. # | Size | Qty. | Price |
|---|---|---|---|
| 11932S | 10 ml |
|
$ 148 |
