This product contains 0.05% sodium azide as a preservative and is stable for 12 months when stored at 4°C. Peroxidase-based fluorescence assays may be negatively affected by sodium azide (1).
This product is supplied as a 1X working solution for antibody dilution in immunofluorescence assays with cell cultures (IF-IC) or frozen tissue samples (IF-F). Cell Signaling Technology recommends using this buffer according to our protocols for IF-approved primary antibodies to ensure accurate and reproducible results. This product contains enough material for 500 assays based on a 100 μl assay volume.