Immunofluorescence Wash Buffer (10X) is for use in immunofluorescence assays with cell cultures (IF-IC) or frozen tissue samples (IF-F). Cell Signaling Technology recommends using this wash buffer according to our protocols for IF-approved primary antibodies to ensure accurate and reproducible results. This product contains enough material for more than 500 assays based on a 100 μl assay volume.1X Wash Buffer contains 137 mM NaCl, 2.6 mM KCl, 10 mM Na2HPO4, 1.76 mM KH2PO4, 0.005% sodium azide, pH 8.0. The product is free of calcium and magnesium salts.
Dilute to a 1X solution using ddH2O. Once diluted to 1X, store at 4°C and use within one month.
Store 10X buffer at room temperature. Undiluted 10X buffer contains 0.05% sodium azide as a preservative and is stable for 12 months. Peroxidase-based fluorescence assays may be negatively affected by sodium azide (1).
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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