Revision 2

#92891

Store at -20C

JC-1

1 mg

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Background

JC-1 is a membrane-permeable cyanine dye used to measure mitochondrial membrane potential. JC-1 is a dual-emission, green-fluorescent dye (emission maximum at approximately 520 nm) at low membrane potential and forms red-fluorescence (emission maximum at 596 nm) at higher membrane potentials (1). Monomeric JC-1 is the predominant form in cells exhibiting lower mitochondrial membrane potential, while aggregates of JC-1 (“J-aggregates”) are found in cells with higher mitochondrial membrane potential (2). JC-1 can be used to detect cells undergoing apoptosis, with healthy cells exhibiting red-fluorescence and cytoplasmic JC-1 exhibiting green-fluorescence in apoptotic cells (3). JC-1 is also a substrate of the multidrug transporter P-glycoprotein (P-gp, MDR1), an ATP-binding cassette transporter protein associated with development of multidrug resistance. Transport of JC-1 by P-gp from cells can reduce cellular JC-1 levels, creating a false indication of decreased mitochondrial membrane potential (4).

Fluorescent Properties

Monomer excitation maximum = 490 nm

Monomer emission maximum = 520 nm

J-aggregate excitation maximum = 535 nm

J-aggregate emission maximum = 596 nm

Molecular Formula

C25H27Cl4IN4

Molecular Weight

652.2 g/mol

Purity

>98%

CAS

3520-43-2

Solubility

Soluble in DMSO at 15 mg/mL or water at 50 mg/mL.

Storage

Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at 4ºC and use within 24 months to prevent loss of potency. Do not freeze/thaw solutions.

Directions for Use

JC-1 is supplied as a lyophilized powder. For a 5 mM stock, reconstitute 1 mg of powder in 0.31 mL of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.