News from the Bench
Discover what’s going on at CST, receive our latest application notes and tips, read our science features, and learn about our products.
the purchase of 3 or more products
To Purchase # 8553S
Find answers on our FAQs page.
- Additional protein information
- Analytical tools
Gallery: PMSF #8553
Phenylmethanesulfonyl Fluoride (PMSF) is an inhibitor of serine proteases such as trypsin, chymotrypsin, thrombin, and papain. It is routinely added as a supplement to lysis buffers just prior to lysis, to prevent protease degradation. Cell Signaling Technology recommends adding PMSF at 1 mM to Cell Lysis Buffer (#9803) and RIPA Buffer (#9806). Molecular Formula: C7H7FO2S Molecular Weight: 174.19 CAS: 329-98-6Molecular Formula:
For a 200 mM (200X) stock solution, reconstitute the 34.84 mg of lyophilized PMSF supplied in the product tube in 1 mL isopropyl alcohol. PMSF is unstable in water and should be added to lysis buffers or other aqueous solutions just prior to use. Typically, a final concentration of 1 mM provides sufficient protease protection. Store lyophilized at RT for 12 months protected from direct light. Once in solution, PMSF can be stored at -20ºC for up to 3 months, protected from light.Storage: Store at Room Temperature
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.