Following immunostaining, cover samples with ProLong® Gold Antifade Reagent and coverslip.
To prevent fading, allow slides to cure in the dark, at room temperature for 24 hours, then seal the coverslips with clear lacquer, such as nail polish.
Proceed with imaging.
Store at -20°C. ProLong® Gold Antifade Reagent is stable for 12 months when stored, protected from light, at or below -20°C.
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