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Tris Buffered Saline (TBS-10X)
Buffers & Dyes

Tris Buffered Saline (TBS-10X) #12498

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Product Usage Information

Dilute Tris Buffered Saline (TBS-10X) to a 1X solution using ddH2O. This product supplies enough 10X material to make 10 liters of 1X solution.


Store 10X buffer at room temperature. At 10X, this buffer is stable for 24 months. Use within 1 week once it has been diluted to 1X.

Product Description

Tris buffered saline (TBS) solution for use as the blocking buffer diluent during fluorescent western blotting. Tween 20® detergent cannot be present in the blocking buffer because it can auto-fluoresce and increase non-specific background. After the blocking step, Tween 20® can be re-introduced to subsequent diluent buffers. Chemliluminescent western blotting does not require Tween 20® omission during blocking. Product is shipped and stored at room temperature.
1X Formulation: 137 mM Sodium Chloride, 20 mM Tris. Supplied at pH 7.6.
NOTE: This product does not contain Tween 20® detergent.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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To Purchase # 12498
Cat. # Size Qty. Price
1 liter
$ 68