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83323
CellSimple™ Cell Health Assay Kit
Assay Kit

CellSimple Cell Health Assay Kit #83323

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CellSimple cell-based analysis of Jurkat cells, untreated (left panel) or treated with Staurosporine #9953 (10 μM, 18 hr; right panel) using the CellSimple Cell Health Assay Kit. Data was collected in both red (561 nm LP; y-axis) and green (525/45 nm; x-axis) channels and analyzed on the Cell Health application. Note the shift from high mean fluorescence intensity (MFI) in the green channel (untreated live cells) to high MFI in the red channel (staurosporine-treated, dead cells). Instrument screen shots are shown.
Graphical representation of the live and dead cell populations in untreated (left panel) or Staurosporine treated (10 μM, 18 hr; right panel) Jurkat cells. Analysis was performed using the Cell Health application and is based on the measurements obtained from the four quadrants of the dot blot figure shown on the previous page. Instrument screen shots are shown.
Inquiry Info.# 83323
Product Includes Quantity (with Count) Solution Color
Calcein AM 1 x 40 µg
Propidium Iodide (PI) Solution 1 x 200 µl
Phosphate Buffered Saline (PBS-20X) 9808 1 x 25 ml

Product Description

The CellSimple Cell Health Assay Kit is a fluorescent assay designed for use with the CellSimple Cell Analyzer. The Cell Health Assay can determine cell viability by measuring intracellular esterase activity and plasma membrane integrity. The assay kit contains the fluorescent dyes Calcein-AM and Propidium Iodide (PI) and is therefore able to stain both live and dead cells simultaneously.

Specificity / Sensitivity

Species Reactivity:

Human

Species predicted to react based on 100% sequence homology:

All Species Expected

Background

The CellSimple Cell Analyzer is a benchtop instrument that utilizes a disposable thin-film cassette and a combination of a 488 nm laser, two photomultiplier tubes (525/45 nm and 561 nm LP filters), Coulter Principle-based cell measurements, and on-board software to provide easy-to-run applications and data analysis. Data acquisition occurs within approximately 10 seconds per test. The instrument relies on disposable cassettes for sample handling, which alleviates the need for flow cell cleaning and fluidics maintenance and the instrument is small enough to be portable between the lab bench and the hood. Applications include quantitative assessments of cell viability, apoptosis, other labeled antibody markers and single and multiplexed bead-based assays for protein and cellular analysis.
Measures of cell viability and cytotoxicity are broadly used to study the effects of growth factors and cytokines, inhibitors and activators, and immune response signals. Calcein-AM is the acetomethoxyl form of calcein, a highly lipophilic, cell membrane permeable dye. Intracellular esterase activity converts the non-fluorescent Calcein-AM to the highly fluorescent Calcein, which is retained only within live cells (1,2). The DNA-binding agent PI is cell membrane impermeable and only enters dead cells or those with damaged cell membranes. Intracellular PI binds DNA and undergoes an approximate 40-fold enhancement in fluorescence intensity. As a result, live cells will produce a strong green fluorescence resulting from the conversion of Calcein-AM to Calcein, while dead cells produce a strong red fluorescence due to the presence of PI (3,4). These fluorescent signals can be detected using the Cell Health application on the CellSimple Cell Analyzer.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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