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29213
CellSimple™ Cellular Stress Antibody Assay Kit
Assay Kit

CellSimple Cellular Stress Antibody Assay Kit #29213

Citations (0)
CellSimple cell-based analysis of untreated (left) or UV treated (right) HeLa cells using CellSimple Cellular Stress Antibody Assay Kit. Data was collected in both green (525/45 nm) and red (561 nm LP) channels and analyzed on the Open Flow application. Dot plots represent mean fluorescence intensity (MFI) of Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (Alexa Fluor® 488 Conjugate) #9719 (x-axis) and Phospho p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb (PE Conjugate) #6908 (y-axis). Instrument screen shots are shown.
Inquiry Info.# 29213

Product Description

The CellSimple Cellular Stress Antibody Assay Kit is designed for use with the CellSimple Cell Analyzer. The kits allows for the quick and easy measurement of the extent of cellular stress or DNA damage under given experimental conditions.

Specificity / Sensitivity

Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb (PE conjugate) recognizes endogenous levels of p38 MAPK only when dually phosphorylated at
Thr180/Tyr182. This antibody does not cross-react with other MAPK family members. Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes H2A.X only when phosphorylated at Ser139. This antibody does not cross react with other phosphorylated histone proteins.

Species Reactivity:

Human, Mouse

Background

The CellSimple Cell Analyzer is a benchtop instrument that utilizes a disposable thin-film cassette and a combination of a 488 nm laser, two photomultiplier tubes (525/45 nm and 561 nm LP filters), Coulter Principle-based cell measurements, and on-board software to provide easy-to-run applications and data analysis. Data acquisition occurs within approximately 10 seconds per test. The instrument relies on disposable cassettes for sample handling, which alleviates the need for flow cell cleaning and fluidics maintenance and the instrument is small enough to be portable between the lab bench and the hood. Applications include quantitative assessments of cell viability, apoptosis, other labeled antibody markers and single and multiplexed bead-based assays for protein and cellular analysis.
p38 MAP kinase (MAPK) is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors. p38 MAPK is activated by phosphorylation at Thr180 and Tyr182 by various MAPK kinases. Once activated the p38 MAPK phosphorylates a wide spectrum of cellular targets to help the cells adapt and cope with noxious conditions (1). Histone H2A.X is required for checkpoint-mediated cell cycle arrest and DNA repair following double-stranded DNA breaks. DNA damage, caused by ionizing radiation, UV-light, or radiomimetic agents results in rapid phosphorylation of H2A.X at Ser139. This very early event in the DNA-damage response is required for recruitment of a multitude of DNA-damage response proteins. In addition to its role in DNA-damage repair, H2A.X is required for DNA fragmentation during apoptosis and is phosphorylated by various kinases in response to apoptotic signals (2).

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
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