#Q8IW41
8550
Product Information
Storage
Store at –80°C.
Keep enzymes on ice during use.
Avoid repeated freeze-thaw cycles.
Source / Purification
The GST-Kinase fusion protein was produced using a baculovirus expression system with a construct expressing human MAPKAPK-5 (Met1-Gln473) (GenBank Accession No. NM_139078) with an amino-terminal GST tag. The protein was purified by one-step affinity chromatography using glutathione-agarose.
Product Description
| Molecular Formula | Peptide substrate, Biotin- Tyrosine Hydroxylase (Ser40): 2,326 Daltons. GST-MAPKAPK-5 Kinase: 84 kDa. |
Background
MAPKAPK-5 belongs to the mitogen-activated protein kinase (MAPK) activated protein kinases (MK) subfamily that includes MAPKAPK-2/MK2 and MK3/3pK. The MK subfamily is part of a family of protein kinase subfamilies downstream of MAPK pathways and includes ribosomal S6 kinase (RSKs), mitogen and stress activated kinases (MSKs) and MAPK-interacting kinases (MNKs). All MKs are activated by MAPK pathways and mediate important processes (e.g. gene expression, cell cycle progression) and have been implicated in inflammation and cancer (1,2). MAPKAPK-5 shows binding to and activation by p38 MAPK and extracellular-regulated kinases (Erk) (3,4). MAPKAPK-5 was shown to be activated by Erk3 and act as a chaperone to Erk3 (5,6). While overexpressed MAPKAPK-5 shares similar substrates with MAPKAPK-2, such as HSP27 and glycogen synthase, recent work with MAPKAPK-5 knock-out mice indicates distinct substrates and functional properties (7).
- Roux, P.P. and Blenis, J. (2004) Microbio.l Mol. Biol. Rev. 68, 320-344.
- Gaestel, M. (2006) Nat. Rev. Mol. Cell Biol. 7, 120-130.
- New, L. et al. (1998) EMBO J. 17, 3372-3384.
- Ni, H. et al. (1998) Biochem. Biophys. Res. Commun. 243, 492-496.
- Schumacher, S. et al. (2004) EMBO J. 23, 4770-4779.
- Seternes, O.M. et al. (2004) EMBO J. 23, 4780-4791.
- Shi, Y. et al. (2003) Mol. Cell Biol. 23, 7732-7741.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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