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7660
HTScan® MAPKAPK-5 Kinase Assay Kit

HTScan® MAPKAPK-5 Kinase Assay Kit #7660

This product is discontinued

Kinase Assay - Radiometric Image 1

Figure 1. MAPKAPK-5 kinase activity was measured in a radiometric assay using the following reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP, variable, 2.5 µg/50 µl PEG20.000, Substrate: Tetra (LRRWSLG), 3 µg/50 µl and recombinant MAPKAPK-5: 200 ng/50 µl.

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Product Includes Quantity (with Count) Solution Color
Kinase Buffer (10X) 9802 1 x 15 ml
ATP (10 mM) 9804 1 x 1 ml

The kit provides a means of performing kinase activity assays with recombinant human MAPKAPK-5 kinase. It includes active MAPKAPK-5 kinase (supplied as a GST fusion protein), a biotinylated peptide substrate and a phospho-serine/threonine antibody for detection of the phosphorylated form of the substrate peptide.

Molecular Formula:

Peptide substrate, Biotin- Tyrosine Hydroxylase (Ser40): 2,326 Daltons. GST-MAPKAPK-5 Kinase: 84 kDa.

Peptide Core Sequence:

RRQS*LIE

MAPKAPK-5 belongs to the mitogen-activated protein kinase (MAPK) activated protein kinases (MK) subfamily that includes MAPKAPK-2/MK2 and MK3/3pK. The MK subfamily is part of a family of protein kinase subfamilies downstream of MAPK pathways and includes ribosomal S6 kinase (RSKs), mitogen and stress activated kinases (MSKs) and MAPK-interacting kinases (MNKs). All MKs are activated by MAPK pathways and mediate important processes (e.g. gene expression, cell cycle progression) and have been implicated in inflammation and cancer (1,2). MAPKAPK-5 shows binding to and activation by p38 MAPK and extracellular-regulated kinases (Erk) (3,4). MAPKAPK-5 was shown to be activated by Erk3 and act as a chaperone to Erk3 (5,6). While overexpressed MAPKAPK-5 shares similar substrates with MAPKAPK-2, such as HSP27 and glycogen synthase, recent work with MAPKAPK-5 knock-out mice indicates distinct substrates and functional properties (7).

  1. Roux, P.P. and Blenis, J. (2004) Microbiol Mol Biol Rev 68, 320-44.
  2. Gaestel, M. (2006) Nat. Rev. Mol. Cell Biol. 7, 120-130.
  3. New L et al. (1998) EMBO J 17, 3372–84
  4. Ni, H. et al. (1998) Biochem. Biophys. Res. Commun. 243, 492-496.
  5. Schumacher, S. et al. (2004) EMBO J. 23, 4770-4779.
  6. Seternes, O.M. et al. (2004) EMBO J. 23, 4780-4791.
  7. Shi, Y. et al. (2003) Mol. Cell Biol. 23, 7732-7741.
Entrez-Gene Id
8550
Swiss-Prot Acc.
Q8IW41
For Research Use Only. Not For Use In Diagnostic Procedures.

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