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PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools


Product Includes Quantity (with Count) Solution Color
DTT (1000X) 1 x 80 µl
ATP (10 mM) 9804 1 x 1 ml

Product Description

The kit provides a means of performing enzymatic assays with active human Lck kinase. It includes active Lck kinase (supplied as a GST fusion protein), a biotinylated substrate peptide and a phospho-tyrosine monoclonal antibody for detection of the phosphorylated form of the substrate peptide.

Molecular Formula:

Peptide substrate, Biotin-FHIT (Tyr114): 2,150 Daltons, GST-Lck Kinase: 84 kDa.

Peptide Core Sequence:


The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).

Lck is essential for T-lymphocyte activation and differentiation (3,4). Phosphorylation of Tyr505 in the carboxy-terminal tail of Lck downregulates its catalytic activity, while phosphorylation of Tyr394 leads to an increase in Lck activity (5).

1.  Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609.

2.  Hunter, T. (1987) Cell 49, 1-4.

3.  Molina, T.J. et al. (1992) Nature 357, 161-164.

4.  Chow, L.M. et al. (1993) Nature 365, 156-60.

5.  Straus, D.B. and Weiss, A. (1992) Cell 70, 585-93.

Entrez-Gene Id 3932
Swiss-Prot Acc. P06239

Data Sheets & Documentation

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DELFIA is a registered trademark of PerkinElmer, Inc.

HTScan® Lck Kinase Assay Kit