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PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools


Product Includes Quantity (with Count) Solution Color
ATP (10 mM) 9804 1 x 1 ml
DTT (1000X) 1 x 80 µl

Product Description

The kit provides a means of performing kinase activity assays with recombinant human Abl1 T315I kinase. It includes active Abl1 T315I kinase (supplied as a GST fusion protein), a biotinylated peptide substrate and a phospho-tyrosine monoclonal antibody for detection of the phosphorylated form of the substrate peptide.

Molecular Formula:

Peptide substrate, Biotin-Signal Transduction Protein (Tyr160): 1830 Daltons. GST-Abl1 T315I Kinase: 76 kDa.

Peptide Core Sequence:


The c-Abl proto-oncogene encodes a nonreceptor protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells. It is implicated in regulating cell proliferation, differentiation, apoptosis, cell adhesion, and stress responses (1-3). c-Abl kinase activity is increased in vivo by diverse physiological stimuli including integrin activation; PDGF stimulation; and binding to c-Jun, Nck, and RFX1 (2,4). The in vivo mechanism for regulation of c-Abl kinase activity is not completely understood. Tyr245 is located in the linker region between the SH2 and catalytic domains. This positioning is conserved among Abl family members. Phosphorylation at Tyr245 is involved in the activation of c-Abl kinase (5). In addition, phosphorylation at Tyr412, which is located in the kinase activation loop of c-Abl, is required for kinase activity (6).

Imatinib (Gleevec) has been successfully used to treat Bcr-Abl kinase associated chronic myeloid leukemia (CML). The most frequently identified mutation associated with resistance to Gleevec in CML patients is T315I in the Abl kinase domain (7,8).

1.  Wang, J.Y. et al. (2000) Oncogene 19, 5643-5650.

2.  Van Etten, R.A. et al. (1999) Trends Cell. Biol. 9, 179-182.

3.  Danial, N.N. et al. (2000) Oncogene 19, 2523-2531.

4.  Shaul, Y. et al. (2000) Cell Death Differ. 7, 10-16.

5.  Brasher, B.B. et al. (2000) J. Biol. Chem. 275, 35631-35637.

6.  Pluk, H. et al. (2002) Cell 108, 247-259.

7.  Fabian, M.A. et al. (2005) Nat. Biotechnol. 23, 329-36.

8.  Carter, T.A. et al. (2005) Proc. Natl. Acad. Sci. USA 102, 11011-6.

Entrez-Gene Id 25
Swiss-Prot Acc. P00519

Data Sheets & Documentation

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DELFIA is a registered trademark of PerkinElmer, Inc.

HTScan® Abl1 T315I Kinase Assay Kit