|40295S||1 Kit (100 assays)||
|Product Includes||Quantity (with Count)|
|Annexin V-PE Conjugate||1 x 200 µl|
|10X Annexin V Binding Buffer||2 x 1.5 ml|
|7-AAD Cell Staining Solution 47501||1 x 1 ml|
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
posted May 2019
Protocol Id: 1824
All Species Expected
Annexin V belongs to the annexin family of proteins consisting of over 160 members. All annexin family members share the same characteristic of Ca2+-dependent binding to negatively charged phospholipid surfaces (1). During early apoptosis, phosphatidylserine (PS) is translocated from the cytosolic side of the plasma membrane to the cellular surface. This translocation exposes PS to the extracellular environment with the plasma membrane left intact (2). Annexin V protein demonstrates high affinity, specificity, and sensitivity for PS and can be used as a marker of early apoptosis. In order to rule out "leaky" necrotic cells, annexin V must be used in tandem with reagents that determine the integrity of the cell membrane, such as propidium iodide (2).
UniProt ID: P08758
Entrez-Gene Id: 308
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