|6813S||1 Kit (500 assays (96 well format))||
|Product Includes||Quantity (with Count)||Solution Color|
|BrdU||1 x 150 µl|
|Fixing/denaturing Solution||2 x 25 ml|
|BrdU Mouse Detection mAb||1 x 500 µl||Green|
|Anti-mouse IgG, HRP-Linked Antibody||1 x 500 µl||Red|
|Detection Antibody Diluent||1 x 50 ml||Green|
|HRP Diluent||1 x 50 ml||Red|
|20X Wash Buffer||1 x 50 ml|
|TMB Substrate 7004||1 x 50 ml|
|STOP Solution 7002||2 x 25 ml|
Incubate for 30 min at room temperature.
NOTE: Watch the color change as it may be necessary to stop the reaction prior to the standard development time of 30 min.
posted October 2012
Protocol Id: 60
Halogenated nucleotides such as the pyrimidine analog bromodeoxyuridine (BrdU) are useful for labeling nascent DNA in living cells and tissues. BrdU becomes incorporated into replicating DNA in place of thymidine and subsequent immunodetection of BrdU using specific monoclonal antibodies allows labeling of cells in S phase of the cell cycle. After pulse-labeling cells or tissues with bromodeoxyuridine, BrdU (Bu20a) Mouse mAb can be used to detect BrdU incorporated into single stranded DNA. Please see our detailed protocol for information regarding the labeling procedure and denaturation of double stranded DNA for various immunodetection applications (1-4).
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